CD133 antibody (C-Term)

Catalog No. ABIN7686775

This anti-CD133 antibody is a Rabbit Polyclonal antibody detecting CD133 in ICC, IF, WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for CD133 antibody (C-Term) (ABIN7686775)

Target: CD133 (PROM1) (Prominin 1 (PROM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD133 antibody is un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), ELISA

Product Details anti-CD133 Antibody

Binding Specificity: C-Term

Specificity: PROM1 antibody is human, mouse and rat reactive. Multiple isoforms of PROM1 are known to exist.

Purification: PROM1 antibody is affinity chromatography purified via peptide column.

Immunogen: PROM1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PROM1. The immunogen is located within the last 50 amino acids of PROM1.

Isotype: IgG

Application Details

Application Notes: PROM1 antibody can be used for detection of PROM1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunocytochemistry at 2.5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PROM1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PROM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for CD133

Target: CD133 (PROM1) (Prominin 1 (PROM1))

Alternative Name: PROM1

Background: PROM1 is a pentaspan transmembrane glycoprotein that localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation (1). Mutations in this gene have been shown to result in retinitis pigmentosa (2). Expression of this gene is also associated with several types of cancer (3).

Molecular Weight: Predicted: 95 kDa

Observed: 97 kDa

Gene ID: 8842

NCBI Accession: NP_006008

UniProt: O43490