Beclin 2 antibody (N-Term)

Catalog No. ABIN6992071

This Rabbit Polyclonal antibody specifically detects Beclin 2 in WB, IHC (p), ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for Beclin 2 antibody (N-Term) (ABIN6992071)

Target: Beclin 2 (BECN1L1) (Beclin-1-Like Protein 1 (BECN1L1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunofluorescence (IF)

Product Details anti-Beclin 2 Antibody

Binding Specificity: AA 30-80, N-Term

Specificity: Beclin 2 antibody is human specific. At least two isoforms of Beclin 2 are known to exist, this antibody will only detect the longer isoform. Beclin 2 antibody is predicted to not cross-react with Beclin 1.

Purification: Beclin 2 antibody is affinity chromatography purified via peptide column.

Immunogen: Beclin 2 antibody was raised against a 16 amino acid peptide near the amino terminus of human Beclin 2. The immunogen is located within amino acids 30 - 80 of Beclin 2.

Isotype: IgG

Application Details

Application Notes: Beclin 2 antibody can be used for detection of Beclin 2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Beclin 2 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Beclin 2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Beclin 2

Target: Beclin 2 (BECN1L1) (Beclin-1-Like Protein 1 (BECN1L1))

Alternative Name: Beclin 2

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin 2 is a mammalian specific homolog of the autophagy protein Beclin 1 (3). Like Beclin 1, Beclin 2 interacts with Bcl-2 and class III PI3K complex components. However, Beclin 2 functions in an additional lysosomal degradation pathway and is required for ligand-induced endolysosomal degradation of several G protein-coupled receptors (3). Beclin 2 is also required for agonist-induced lysosome-mediated degradation of EGFR in lung cancer cells, suggesting that it may also play a role in regulating other intracellular signaling pathways (4).

Molecular Weight: Predicted: 47 kDa

Observed: 55 kDa

Gene ID: 441925

NCBI Accession: NP_001277622

UniProt: A8MW95