ARID1A antibody (N-Term)

Catalog No. ABIN6992134

This Rabbit Polyclonal antibody specifically detects ARID1A in ELISA, WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ARID1A antibody (N-Term) (ABIN6992134)

Target: ARID1A (AT Rich Interactive Domain 1A (SWI-Like) (ARID1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID1A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARID1A Antibody

Binding Specificity: AA 580-630, N-Term

Specificity: ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.

Purification: ARID1A antibody is affinity chromatography purified via peptide column.

Immunogen: ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A. The immunogen is located within amino acids 580 - 630 of ARID1A.

Isotype: IgG

Application Details

Application Notes: ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ARID1A antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ARID1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for ARID1A

Target: ARID1A (AT Rich Interactive Domain 1A (SWI-Like) (ARID1A))

Alternative Name: ARID1A

Background: The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).

Molecular Weight: Predicted: 133, 227, 251 kDa

Observed: 130, 260 kDa

Gene ID: 8289

NCBI Accession: NP_006006

UniProt: O14497

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Tube Formation