P4HB antibody (AA 190-240)

Catalog No. ABIN6992148

This anti-P4HB antibody is a Rabbit Polyclonal antibody detecting P4HB in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for P4HB antibody (AA 190-240) (ABIN6992148)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-P4HB Antibody

Binding Specificity: AA 190-240

Specificity: PDIA1 antibody is human, mouse and rat reactive.

Purification: PDIA1 antibody is affinity chromatography purified via peptide column.

Immunogen: PDIA1 antibody was raised against a 17 amino acid peptide near the center of human PDIA1. The immunogen is located within amino acids 190 - 240 of PDIA1.

Isotype: IgG

Application Details

Application Notes: PDIA1 antibody can be used for detection of PDIA1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PDIA1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PDIA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDIA1

Background: PDIA1 (protein disulfide isomerase family A member 1) is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen (1,2). PDIA1 is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds (3). Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (4).

Molecular Weight: Predicted: 54 kDa

Observed: 54 kDa

Gene ID: 5034

NCBI Accession: NP_000909

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism