BCLAF1 antibody (C-Term)

Catalog No. ABIN6992173

Product Details anti-BCLAF1 Antibody

Target: BCLAF1 (BCL2-Associated Transcription Factor 1 (BCLAF1))

Binding Specificity: AA 820-870, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCLAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: BCLAF1 antibody is human, mouse and rat reactive. At least four isoforms of BCLAF1 are known to exist, this antibody will detect all but isoform 2.

Purification: BCLAF1 antibody is affinity chromatography purified via peptide column.

Immunogen: BCLAF1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human BCLAF1. The immunogen is located within amino acids 820 - 870 of BCLAF1.

Isotype: IgG

Application Details

Application Notes: BCLAF1 antibody can be used for detection of BCLAF1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: BCLAF1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: BCLAF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for BCLAF1

Target: BCLAF1 (BCL2-Associated Transcription Factor 1 (BCLAF1))

Alternative Name: BCLAF1 (BCLAF1 Products)

Background: The BCL2-associated transcription factor 1 (BCLAF1) is a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins (1). BCLAF1 localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. (2). More recent studies have suggested BCLAF1 plays roles in processes not associated with actions of the BCL2 family members, including lung development, T cell activation, and post-transcriptional processes that affect mRNA metabolism (3).

Molecular Weight: Predicted: 101 kDa

Observed: 105 kDa

Gene ID: 9774

NCBI Accession: NP_055554

UniProt: Q9NYF8

Pathways: Positive Regulation of Response to DNA Damage Stimulus