PD-L1 antibody (AA 34-241)

Catalog No. ABIN7428623

This Mouse Monoclonal antibody specifically detects PD-L1 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for PD-L1 antibody (AA 34-241) (ABIN7428623)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: D6

Product Details anti-PD-L1 Antibody

Binding Specificity: AA 34-241

Purpose: Monoclonal Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)

Specificity: The antibody is a mouse monoclonal antibody raised against PDL1. It has been selected for its ability to recognize PDL1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Programmed Cell Death Protein 1 Ligand 1 (PDL1) corresdonding to Ser34~Val241 with N-terminal His and GST Tag

Isotype: IgG2a kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000,Immunohistochemistry: 5-20 μg/mL,1:50-200,Immunocytochemistry: 5-20 μg/mL,1:50-200,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PDL1

Background: CD274, PDCD1LG1, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1-LG1, Programed Death Ligand 1

UniProt: D4AE25

Pathways: Cancer Immune Checkpoints