alpha 2 Macroglobulin antibody (AA 216-437)

Catalog No. ABIN7427846

This anti-alpha 2 Macroglobulin antibody is a Mouse Monoclonal antibody detecting alpha 2 Macroglobulin in WB, IHC, IP and ICC. Suitable for Rat.

Quick Overview for alpha 2 Macroglobulin antibody (AA 216-437) (ABIN7427846)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: A1

Product Details anti-alpha 2 Macroglobulin Antibody

Binding Specificity: AA 216-437

Purpose: Monoclonal Antibody to Alpha-2-Macroglobulin (a2M)

Specificity: The antibody is a mouse monoclonal antibody raised against a2M. It has been selected for its ability to recognize a2M in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Alpha-2-Macroglobulin (a2M) corresdonding to Arg216~Phe437 (Accession # P06238)

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-30 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL,Immunohistochemistry in paraffin section: 5-30 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: Alpha-2-Macroglobulin

Background: A2MG, A2 mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

UniProt: P06238

Pathways: Lipid Metabolism