beta Actin antibody (AA 1-375)

Catalog No. ABIN7428920

The Mouse Monoclonal anti-beta Actin antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect beta Actin in samples from Human, Mouse, Rat, Dog, Pig and Cow.

Quick Overview for beta Actin antibody (AA 1-375) (ABIN7428920)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C5

Product Details anti-beta Actin Antibody

Binding Specificity: AA 1-375

Purpose: Monoclonal Antibody to Beta Actin (ACTB)

Specificity: The antibody is a mouse monoclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.

Cross-Reactivity: Rabbit

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Beta Actin (ACTB) corresdonding to Met1~Phe375 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background: Actin Beta, ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction