ENO1 antibody (AA 1-434)

Catalog No. ABIN7426280

This Mouse Monoclonal antibody specifically detects ENO1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for ENO1 antibody (AA 1-434) (ABIN7426280)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C2

Product Details anti-ENO1 Antibody

Binding Specificity: AA 1-434

Purpose: Monoclonal Antibody to Enolase 1 (ENO1)

Specificity: The antibody is a mouse monoclonal antibody raised against ENO1. It has been selected for its ability to recognize ENO1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Cow, Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Enolase 1 (ENO1) corresdonding to Met1~Lys434 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: ENO1

Background: NNE, ENO1L1, MPB1, PPH, Alpha Enolase, Enolase 1, Phosphopyruvate hydratase, Plasminogen-binding protein, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding

UniProt: P06733