ECF antibody (AA 24-97)

Catalog No. ABIN7430438

This anti-ECF antibody is a Rabbit Polyclonal antibody detecting ECF in WB, IHC, IP and ICC. Suitable for Rat.

Quick Overview for ECF antibody (AA 24-97) (ABIN7430438)

Target: ECF (Eosinophil Chemotactic Factor (ECF))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ECF Antibody

Binding Specificity: AA 24-97

Purpose: Polyclonal Antibody to Eosinophil Chemotactic Factor (ECF)

Specificity: The antibody is a rabbit polyclonal antibody raised against ECF. It has been selected for its ability to recognize ECF in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Eosinophil Chemotactic Factor (ECF) corresdonding to His24~Pro97 (Accession # P97545) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ECF

Target: ECF (Eosinophil Chemotactic Factor (ECF))

Alternative Name: Eosinophil Chemotactic Factor

Background: CCL11, SCYA11, Eotaxin 1, Eosinophil Chemotactic Protein 1, Chemokine C-C-Motif Ligand 11, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11

UniProt: P97545