alpha Fetoprotein antibody (AA 263-386)

Catalog No. ABIN7438724

The Rabbit Polyclonal anti-alpha Fetoprotein antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect alpha Fetoprotein in samples from Pig.

Quick Overview for alpha Fetoprotein antibody (AA 263-386) (ABIN7438724)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-alpha Fetoprotein Antibody

Binding Specificity: AA 263-386

Purpose: Polyclonal Antibody to Alpha-Fetoprotein (AFP)

Specificity: The antibody is a rabbit polyclonal antibody raised against AFP. It has been selected for its ability to recognize AFP in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Alpha-Fetoprotein (AFP) corresdonding to Ala263~Ser386 (Accession # Q8MJ76) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: Alpha-Fetoprotein

Background: AFP, A-FP, FETA, HPAFP, Alpha-Fetoglobulin, Alpha-1-fetoprotein

UniProt: Q8MJ76

Pathways: C21-Steroid Hormone Metabolic Process