Caspase 12 antibody (AA 1-244)

Catalog No. ABIN7440263

This Rabbit Polyclonal antibody specifically detects Caspase 12 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for Caspase 12 antibody (AA 1-244) (ABIN7440263)

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Caspase 12 Antibody

Binding Specificity: AA 1-244

Purpose: Polyclonal Antibody to Caspase 12 (CASP12)

Specificity: The antibody is a rabbit polyclonal antibody raised against CASP12. It has been selected for its ability to recognize CASP12 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Caspase 12 (CASP12) corresdonding to Met1~Thr244 (Accession # Q920D5)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:370-1400,Immunohistochemistry: 5-20 μg/mL,1:37-140,Immunocytochemistry: 5-20 μg/mL,1:37-140,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.74 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Alternative Name: CASP12

Background: CASP12P1, Cysteinyl Aspartate Specific Proteinases 12, Apoptosis-Related Cysteine Peptidase

UniProt: Q920D5

Pathways: Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response