PD-L1 antibody (AA 34-241)

Catalog No. ABIN7443125

The Rabbit Polyclonal anti-PD-L1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PD-L1 in samples from Rat.

Quick Overview for PD-L1 antibody (AA 34-241) (ABIN7443125)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PD-L1 Antibody

Binding Specificity: AA 34-241

Purpose: Polyclonal Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDL1. It has been selected for its ability to recognize PDL1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Programmed Cell Death Protein 1 Ligand 1 (PDL1) corresdonding to Ser34~Val241 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PDL1

Background: CD274, PDCD1LG1, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1-LG1, Programed Death Ligand 1

UniProt: D4AE25

Pathways: Cancer Immune Checkpoints