DLD antibody (AA 45-271)

Catalog No. ABIN7432892

The Rabbit Polyclonal anti-DLD antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect DLD in samples from Mouse.

Quick Overview for DLD antibody (AA 45-271) (ABIN7432892)

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-DLD Antibody

Binding Specificity: AA 45-271

Purpose: Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)

Specificity: The antibody is a rabbit polyclonal antibody raised against DLD. It has been selected for its ability to recognize DLD in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Dihydrolipoyl Dehydrogenase (DLD) corresdonding to Thr45~Lys271 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Alternative Name: Dihydrolipoyl Dehydrogenase

Background: LAD, GCSL, Dihydrolipoamide Dehydrogenase, E3 Component Of Pyruvate Dehydrogenase Complex, 2-Oxo-Glutarate Complex, Branched Chain Keto Acid Dehydrogenase Complex

UniProt: O08749

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis