GCLM antibody (AA 40-251)

Catalog No. ABIN7433051

The Rabbit Polyclonal anti-GCLM antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect GCLM in samples from Human.

Quick Overview for GCLM antibody (AA 40-251) (ABIN7433051)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-GCLM Antibody

Binding Specificity: AA 40-251

Purpose: Polyclonal Antibody to Glutamate Cysteine Ligase, Modifier Subunit (GCLM)

Specificity: The antibody is a rabbit polyclonal antibody raised against GCLM. It has been selected for its ability to recognize GCLM in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glutamate Cysteine Ligase, Modifier Subunit (GCLM) corresdonding to Ser40~Ser251 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000,Immunohistochemistry: 5-20 μg/mL,1:50-200,Immunocytochemistry: 5-20 μg/mL,1:50-200,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GCLM

Background: GLCLR, GECS, GCL, Gamma Glutamylcysteine Synthetase, Glutamate Cysteine Ligase Regulatory, Gamma-ECS regulatory subunit, Glutamate cysteine ligase modifier subunit

UniProt: P48507