KLF4 antibody (AA 240-478)

Catalog No. ABIN7440023

This Rabbit Polyclonal antibody specifically detects KLF4 in WB, IHC, ICC and IP. It exhibits reactivity toward Mouse.

Quick Overview for KLF4 antibody (AA 240-478) (ABIN7440023)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-KLF4 Antibody

Binding Specificity: AA 240-478

Purpose: Polyclonal Antibody to Kruppel Like Factor 4, Gut (KLF4)

Specificity: The antibody is a rabbit polyclonal antibody raised against KLF4. It has been selected for its ability to recognize KLF4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Kruppel Like Factor 4, Gut (KLF4) corresdonding to Ser240~His478 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background: EZF, GKLF, Gut-Enriched Krüppel-Like Factor, Epithelial zinc finger protein EZF

UniProt: Q60793

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance