15-LO-2 (AA 349-633) antibody
Reactivity: Rat
ICC, IHC, IP, WB
Host: Rabbit
Polyclonal
unconjugated
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- Target
- 15-LO-2
- Binding Specificity
- AA 349-633
- Reactivity
- Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
- Application
- Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Polyclonal Antibody to 15-Lipoxygenase-2 (15-LO-2)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against 15-LO-2. It has been selected for its ability to recognize 15-LO-2 in immunohistochemical staining and western blotting.
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant 15-Lipoxygenase-2 (15-LO-2) corresdonding to Asp349~Val633 (Accession # Q8K4F2)
- Isotype
- IgG
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anti-15-LO-2 (AA 253-490) antibody
Reactivity: Human ICC, IHC, IP, WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Western blotting: 0.5-2 μg/mL,1:350-1400,Immunohistochemistry: 5-20 μg/mL,1:35-140,Immunocytochemistry: 5-20 μg/mL,1:35-140,Optimal working dilutions must be determined by end user.
- Comment
-
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- ProClin, Sodium azide
- Precaution of Use
- This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
-
- Target
- 15-LO-2
- Background
- ALOX15B, LOX15B, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Lipoxygenase 15, Type B, 15-lipoxygenase 2, Linoleate 13-lipoxygenase 15-LOb Arachidonate-15-Lipoxygenase, Type B
- UniProt
- Q8K4F2
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