CYP21A2 antibody (AA 106-389)

Catalog No. ABIN7441886

Product Details anti-CYP21A2 Antibody

Target: CYP21A2 (Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2))

Binding Specificity: AA 106-389

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP21A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2)

Specificity: The antibody is a rabbit polyclonal antibody raised against CYP21A2. It has been selected for its ability to recognize CYP21A2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) corresdonding to Asp106~Gln389 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CYP21A2

Target: CYP21A2 (Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2))

Alternative Name: Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2 Products)

Background: CYP21B, CPS1, CA21H, CAH1, CYP21, CYP21-B, 21-OHase, Cytochrome P450 21B, Steroid 21-hydroxylase, Cytochrome P450,Family 21,Subfamily A,Polypeptide 2

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process