CD3D antibody (AA 19-99)

Catalog No. ABIN7436930

This Rabbit Polyclonal antibody specifically detects CD3D in WB, IHC, IP and ICC. It exhibits reactivity toward Sheep.

Quick Overview for CD3D antibody (AA 19-99) (ABIN7436930)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CD3D Antibody

Binding Specificity: AA 19-99

Purpose: Polyclonal Antibody to Cluster Of Differentiation 3d (CD3d)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD3d. It has been selected for its ability to recognize CD3d in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cluster Of Differentiation 3d (CD3d) corresdonding to Val19~Thr99 (Accession # P18438) with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000,Immunohistochemistry: 5-20 μg/mL,1:50-200,Immunocytochemistry: 5-20 μg/mL,1:50-200,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3d

Background: CD3-DELTA, T3D, T-Dell Surface Glycoprotein CD3 Delta Chain, T-cell receptor T3 delta chain

UniProt: P18438

Pathways: TCR Signaling, CXCR4-mediated Signaling Events