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PUMA antibody (AA 76-170)

BBC3 Reactivity: Rat WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7434332
  • Target See all PUMA (BBC3) Antibodies
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Binding Specificity
    • 16
    • 13
    • 9
    • 8
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 76-170
    Reactivity
    • 88
    • 39
    • 38
    • 16
    Rat
    Host
    • 84
    • 6
    • 1
    Rabbit
    Clonality
    • 75
    • 16
    Polyclonal
    Conjugate
    • 48
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PUMA antibody is un-conjugated
    Application
    • 72
    • 30
    • 24
    • 22
    • 17
    • 14
    • 13
    • 13
    • 8
    • 7
    • 5
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    Purpose
    Polyclonal Antibody to p53 Upregulated Modulator Of Apoptosis (PUMA)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against PUMA. It has been selected for its ability to recognize PUMA in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Human, Mouse
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant p53 Upregulated Modulator Of Apoptosis (PUMA) corresdonding to Arg76~Val170
    Isotype
    IgG
    Top Product
    Discover our top product BBC3 Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    1:280-1100 Immunohistochemistry: 5-20 μg/mL

    1:28-110 Immunocytochemistry: 5-20 μg/mL

    1:28-110 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Alternative Name
    p53 Upregulated Modulator Of Apoptosis (BBC3 Products)
    Background
    JFY1, BBC3, BCL2 Binding Component 3
    Pathways
    p53 Signaling, Positive Regulation of Endopeptidase Activity
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