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PUMA antibody (BH3 Domain, C-Term)

BBC3 Reactivity: Human, Mouse WB, IF, FACS, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN356830
  • Target See all PUMA (BBC3) Antibodies
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Binding Specificity
    • 20
    • 18
    • 16
    • 9
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    BH3 Domain, C-Term
    Reactivity
    • 113
    • 40
    • 38
    • 16
    Human, Mouse
    Host
    • 109
    • 7
    • 1
    Rabbit
    Clonality
    • 98
    • 19
    Polyclonal
    Conjugate
    • 52
    • 8
    • 8
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PUMA antibody is un-conjugated
    Application
    • 98
    • 40
    • 39
    • 22
    • 21
    • 16
    • 16
    • 14
    • 13
    • 9
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody is specific to Human and Mouse PUMA (BH3 Domain). Other species not tested.
    Purification
    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
    Immunogen
    Synthetic peptide selected from the C-terminal region of Human PUMA
    Isotype
    Ig Fraction
    Top Product
    Discover our top product BBC3 Primary Antibody
  • Application Notes
    ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS containing 0.09 % (W/V) Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Alternative Name
    PUMA (BBC3 Products)
    Synonyms
    BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody
    Background
    PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
    Molecular Weight
    20532 Da
    Gene ID
    27113, 9606
    UniProt
    Q9BXH1
    Pathways
    p53 Signaling, Positive Regulation of Endopeptidase Activity
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