LCAT antibody (AA 41-210)

Catalog No. ABIN7443461

This anti-LCAT antibody is a Rabbit Polyclonal antibody detecting LCAT in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for LCAT antibody (AA 41-210) (ABIN7443461)

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-LCAT Antibody

Binding Specificity: AA 41-210

Purpose: Polyclonal Antibody to Lecithin Cholesterol Acyltransferase (LCAT)

Specificity: The antibody is a rabbit polyclonal antibody raised against LCAT. It has been selected for its ability to recognize LCAT in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Lecithin Cholesterol Acyltransferase (LCAT) corresdonding to Glu41~His210 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for LCAT

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Alternative Name: LCAT

Background: Phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase

UniProt: P04180

Pathways: Lipid Metabolism