FLIP antibody (AA 210-460)

Catalog No. ABIN7439762

This Rabbit Polyclonal antibody specifically detects FLIP in WB, IHC, ICC and IP. It exhibits reactivity toward Mouse.

Quick Overview for FLIP antibody (AA 210-460) (ABIN7439762)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-FLIP Antibody

Binding Specificity: AA 210-460

Purpose: Polyclonal Antibody to CASP8 And FADD Like Apoptosis Regulator (CFLAR)

Specificity: The antibody is a rabbit polyclonal antibody raised against CFLAR. It has been selected for its ability to recognize CFLAR in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant CASP8 And FADD Like Apoptosis Regulator (CFLAR) corresdonding to Asn210~Gly460 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR

Background: CASH, CASP8AP1, CLARP, Casper, FLAME1, FLIP, I-FLICE, MRIT, USURPIN, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, Caspase-eight-related protein, FADD-like antiapoptotic molecule 1

UniProt: O35732

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development