SEMA5A antibody (AA 319-668)

Catalog No. ABIN7430117

The Rabbit Polyclonal anti-SEMA5A antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect SEMA5A in samples from Human.

Quick Overview for SEMA5A antibody (AA 319-668) (ABIN7430117)

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SEMA5A Antibody

Binding Specificity: AA 319-668

Purpose: Polyclonal Antibody to Semaphorin 5A (SEMA5A)

Specificity: The antibody is a rabbit polyclonal antibody raised against SEMA5A. It has been selected for its ability to recognize SEMA5A in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Semaphorin 5A (SEMA5A) corresdonding to Val319~Gln668 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SEMA5A

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Alternative Name: SEMA5A

Background: SEMAF, SemF, Semaphorin-F, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 5A

UniProt: Q13591