SF3B3 antibody (AA 35-210)

Catalog No. ABIN7441685

Product Details anti-SF3B3 Antibody

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Binding Specificity: AA 35-210

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3B3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Splicing Factor 3B Subunit 3 (SF3B3)

Specificity: The antibody is a rabbit polyclonal antibody raised against SF3B3. It has been selected for its ability to recognize SF3B3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Splicing Factor 3B Subunit 3 (SF3B3) corresdonding to Gly35~Phe210 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SF3B3

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Alternative Name: Splicing Factor 3B Subunit 3 (SF3B3 Products)

Background: RSE1, SAP130, SF3b130, STAF130, Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b 130 kDa subunit