ARIH1 antibody

Catalog No. ABIN7003436

This Rabbit Polyclonal antibody specifically detects ARIH1 in ELISA and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for ARIH1 antibody (ABIN7003436)

Target: ARIH1 (Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (ARIH1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARIH1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-ARIH1 Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Synthetic peptide of human ARIH1

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:100, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.9 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ARIH1

Target: ARIH1 (Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (ARIH1))

Alternative Name: ARIH1

Background: ARIH1 (ariadne homolog), also known as ubiquitin conjugating enzyme E2 binding protein 1, ARI, HARI, HHARI (human homolog of Drosophila ariadne), MOP-6 (monocyte protein 6) or UBCH7BP (UBCH7 binding protein), is a 557 amino acid cytoplasmic protein. Expressed in a wide variety of tissues, ARIH1 contains two RING-type zinc fingers and one IBR (in-between RING fingers)-type domain. ARIH1 is believed to be involved in protein degradation and protein translation. ARIH1 interacts with UBCH7 and is thought to function as an E3 ubiquitin-protein ligase (or as a component of an E3 complex) that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme (UBCH7) and transfers that ubiquitin residue to substrates targeted for degradation. Specifically, ARIH1 interacts with and polyubiquitylates eIF4E2, thereby targeting it for proteasomal degradation.

UniProt: Q9Y4X5