PRKAA1/PRKAA2 antibody (pThr172, pThr183)

Catalog No. ABIN7250794

This anti-PRKAA1/PRKAA2 antibody is a Rabbit Polyclonal antibody detecting PRKAA1/PRKAA2 in WB, ELISA, IHC (p) and IF. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for PRKAA1/PRKAA2 antibody (pThr172, pThr183) (ABIN7250794)

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1/PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-PRKAA1/PRKAA2 Antibody

Binding Specificity: pThr172, pThr183

Characteristics: Phosphorylated antibody

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human AMPKalpha1/2 around the phosphorylation site of Thr183/172

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Alternative Name: AMPK alpha1/2

Background: AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.

Molecular Weight:

Observed_MW: 63 kDa

Calculated_MW: 62 kDa

UniProt: Q13131, P54646