IgE antibody (Biotin)

Catalog No. ABIN7013917

The Mouse Monoclonal anti-IgE antibody has been validated for ELISA and FACS. It is suitable to detect IgE in samples from Human.

Quick Overview for IgE antibody (Biotin) (ABIN7013917)

Target: IgE

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgE antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS)

Clone: BE5

Product Details anti-IgE Antibody (Biotin)

Purpose: Anti-Hu IgE Biotin

Specificity: The antibody BE5 reacts with human IgE, it recognizes an epitope different from the ones recognized by 4G7.325 and 4H10 antibodies to IgE.

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Purified human IgE.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-12 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for IgE

Target: IgE

Background: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E