PD-L1 antibody
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- Target See all PD-L1 Antibodies
- PD-L1 (CD274 (PD-L1))
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Reactivity
- Human, Non-Human Primate
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PD-L1 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Anti-Hu CD274 Purified Low Endotoxin
- Specificity
- The mouse monoclonal antibody 29E.2A3 recognizes an extracellular epitope of CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas.
- Purification
- Purified by protein-A affinity chromatography.
- Endotoxin Level
- Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
- Immunogen
- Full length human CD274
- Clone
- 29E-2A3
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product PD-L1 Primary Antibody
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anti-CD274 (PD-L1) (C-Term) antibody
Verified PD-L1 Reactivity: Human WB, FACS, ELISA, IF Host: Goat Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 19-238) antibodyPD-L1 Reactivity: Human WB, FACS, IHC, ELISA, IF Host: Mouse Monoclonal 9B5F4 unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 Reactivity: Human FACS, IHC, ELISA Host: Mouse Monoclonal 6D9A2 unconjugated
anti-CD274 (PD-L1) (AA 39-191) antibodyPD-L1 Reactivity: Human, Mouse WB, FACS, IHC, ELISA, IF, Coat, StM Host: Mouse Monoclonal PDL1-2746 unconjugated
anti-CD274 (PD-L1) (AA 48-290) antibodyPD-L1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 34-241) antibodyPD-L1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 7D2A10 unconjugated
anti-CD274 (PD-L1) (C-Term) antibodyKO Validated PD-L1 Reactivity: Human WB, FACS, IF, ICC, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 201-290) antibodyPD-L1 Reactivity: Mouse, Rat WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-CD274 (PD-L1) antibody (PE)PD-L1 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal 29E-2A3 PE
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- Application Notes
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Functional application: Blocking.
Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: Recommended dilution: 1-4 μg/mL - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- PD-L1 (CD274 (PD-L1))
- Alternative Name
- CD274 (PD-L1 Products)
- Background
- CD274 Molecule,CD274 / PD-L1 (programmed death ligand-1), also known as B7-H1, is a member of the B7 family of regulatory proteins. It can act as both costimulatory and coinhibitory molecule for T cells. Interaction with its receptor CD279 (PD1) appears to be important in the maintenance of peripheral tolerance and in prevention of tumor rejection. Even pathogens (e.g. Schistosoma) may exploit CD274 to evade an immune response. Besides CD279, existence of other receptor(s) for CD274 is likely.,B7H1, PDL1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7-H1
- Gene ID
- 29126
- UniProt
- Q9NZQ7
- Pathways
- Cancer Immune Checkpoints
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