SEMA4D/CD100 antibody

Catalog No. ABIN7014006

This Mouse Monoclonal antibody specifically detects SEMA4D/CD100 in FACS, IP and ELISA. It exhibits reactivity toward Human.

Quick Overview for SEMA4D/CD100 antibody (ABIN7014006)

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SEMA4D/CD100 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA

Clone: 133-1C6

Product Details anti-SEMA4D/CD100 Antibody

Purpose: Anti-Hu CD100 Purified

Specificity: The mouse monoclonal antibody 133-1C6 recognizes an extracellular epitope of CD100, an approximately 150 kDa (when reduced) semaphorin family member expressed mainly on lymphocytes, NK cells, monocytes/macrophages and granulocytes, but also on some non-hematopoietic cells.

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Immunogen: PHA stimulated human PBL

Isotype: IgM

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for SEMA4D/CD100

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name: CD100

Background: Semaphorin 4D,CD100, also known as semaphorin 4D, is a homodimerizing type I transmembrane glycoprotein containing an extracellular semaphorin domain. It is expressed on most hematopoietic cells with the exception of immature bone marrow cells, erythrocytes and platelets. A 120 kDa soluble form is generated from the transmembrane form by proteolytic cascade following primary T and B cell activation. It seems CD100 acts through dampening CD72-mediated negative signaling. CD100 promotes angiogenesis, invasive growth, proliferation and anti-apoptosis of cancer cells in vitro. Higher expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients.,COLL4, SEMA4D, semaphorin 4D

Gene ID: 10507

UniProt: Q92854

Pathways: Regulation of Cell Size