TLN1 antibody (AA 1601-1750) (HRP)

Catalog No. ABIN701704

This Rabbit Polyclonal antibody specifically detects TLN1 in ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for TLN1 antibody (AA 1601-1750) (HRP) (ABIN701704)

Target: TLN1 (Talin 1 (TLN1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLN1 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TLN1 Antibody (HRP)

Binding Specificity: AA 1601-1750

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Talin 1.

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TLN1

Target: TLN1 (Talin 1 (TLN1))

Alternative Name: Talin1

Background:

Synonyms: TLN 1, ILWEQ, Talin 1, Talin, TLN, TLN1, TLN 2, Talin 2, Talin 2, TLN2.

Background: Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.

Gene ID: 7094, 83660

UniProt: Q9Y4G6, Q9Y490

Pathways: Cell-Cell Junction Organization, ER-Nucleus Signaling, Maintenance of Protein Location