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Histone 3 antibody (H3K9me)

Name: Histone 3 antibody (H3K9me)
SKU: ABIN703886
Price: 384.62 USD
Availability: InStock

The Rabbit Polyclonal anti-Histone 3 antibody has been validated for ELISA, ICC, IHC (p), IF (cc), FACS, IF (p) and IHC (fro). It is suitable to detect Histone 3 in samples from Human, Mouse and Rat.

Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-Histone H3 (Di Methyl K9) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (Histone 3 antibody (H3K9me))

IHC (p)

HeLa cells were fixed with 70% ice-cold methanol overnight at 4℃, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. (Histone 3 antibody (H3K9me))

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Histone H3 (Di Methyl K9) Polyclonal Antibody, Unconjugated (ABIN703886) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37 °C and DAPI staining (Histone 3 antibody (H3K9me))

3 images

Catalog No. ABIN703886

Quick Overview for Histone 3 antibody (H3K9me) (ABIN703886)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Reactivity

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Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

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ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product H3 Primary Antibody

Binding Specificity
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H3K9me

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Cow,Pig,Rabbit,Drosophila

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic metylpeptide derived from human Histone H3 around metylation site of Lys9

Isotype

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), IF (cc), FACS, IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

Histone H3

Background

Synonyms: H3F3, H3.3A, Histone H3.3, H3F3A, PP781
Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID

3020

UniProt

P84243