Histone 3 antibody (H3K9ac)

Catalog No. ABIN703946

The Rabbit Polyclonal anti-Histone 3 antibody has been validated for WB, ELISA, IHC (p), FACS, IF (cc), IF (p) and IHC (fro). It is suitable to detect Histone 3 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for Histone 3 antibody (H3K9ac) (ABIN703946)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K9ac

Specificity: This acetylation site is homologous across the species listed.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Rabbit,Drosophila

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys9

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Histone 3 antibody (ABIN703946)

Yeo, Becherel, Luff, Graham, Richard, Lavin: "Senataxin controls meiotic silencing through ATR activation and chromatin remodeling." in: Cell discovery, Vol. 1, pp. 15025, (2016) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background:

Synonyms: H3F3, H3.3A, Histone H3.3, H3F3A, PP781

Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID: 3020

UniProt: P84243