CD73 antibody (Extracellular)

Catalog No. ABIN7042877

Product Details anti-CD73 Antibody

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Binding Specificity: AA 429-433, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to CD73

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - identical

Characteristics: Anti-CD73 (extracellular) Antibody (ABIN7042877, ABIN7044811 and ABIN7044812) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NT5E from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234599)

Blocking Peptide: (ABIN7234599)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: NT5E (NT5E Products)

Background: Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E,CD73 (ecto-5'-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses1,2.CD73 primarily detected in cancer cells and in immune cells such as CD4+Foxp3+ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor2.Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer3.

Alternative names: CD73, Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E

Gene ID: 23959

NCBI Accession: NM_001204813

UniProt: Q61503

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process