ADAM10 antibody (Extracellular)

Catalog No. ABIN7042880

Product Details anti-ADAM10 Antibody

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Binding Specificity: AA 400-414, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to ADAM10 (extracellular)

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-ADAM10 (extracellular) Antibody (ABIN7042880, ABIN7044734 and ABIN7044735)) is a highly specific antibody directed against an extracellular epitope of mouse ADAM10. The antibody can be used in western blot, Indirect flow cytometry and immunohistochemistry. It has been designed to recognize ADAM10 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CTPGESKNLGQKENG, corresponding to amino acid residues 400 - 414 of mouse ADAM10

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:500

Comment:

Negative Control: BLP-NR156

Blocking Peptide: BLP-NR156

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: ADAM10 (ADAM10 Products)

Background: Disintegrin And Metalloproteinase Domain-Containing Protein 10, ADAM Metallopeptidase Domain 10, CD156c,Disintegrin and metalloproteinase domain-containing protein 10, also known as ADAM10, is a membrane-anchored protease that belongs to the ADAM protease family. ADAMs are type I transmembrane proteins with a large extracellular domain and a shorter cytosolic part, they contain a metalloprotease domain, a disintegrin domain, a cystein-rich domain, an EGF-like domain, a transmembrane domain and a cytoplasmic tail2. 37 murine and 22 human ADAMs are known and only some of them contain the catalytically relevant domain (HEXGHXXGXXHD) and are therefore able to cleave the extracellular domains of several membrane-bound proteins in a process known as 'ectodomain shedding'1. This process represents a unique and irreversible posttranslational event regulating the function and half-life of many intracellular and extracellular proteins and hence the ADAM family protein's activity is essential for regulation of various cellular functions2. ADAM10 has many substrates, among which are cellular adhesion molecules such as cadherin, receptors and ligands3. it is also capable of anti-amyloidogenic proteolysis of the amyloid precursor protein, this is why ADAM10 mutations were shown to be correlated with Alzheimer's disease2. In addition, it was shown to involved in various human diseases and disorders such as Huntington's disease, psoriasis, lupus, and multiple types of cancer. The central role of ADAM10 in various cell processes and diseases reveals the potency of ADAM10-targeting drug development as a future therapeutic option2.

Alternative names: Disintegrin And Metalloproteinase Domain-Containing Protein 10, ADAM Metallopeptidase Domain 10, CD156c

Gene ID: 11487

NCBI Accession: NM_001110

UniProt: O35598

Pathways: Notch Signaling, EGFR Signaling Pathway