ADAM10 antibody (C-Term)

Catalog No. ABIN499211

The Rabbit Polyclonal anti-ADAM10 antibody is suitable to detect ADAM10 in samples from Human. It has been validated for WB, IF and EIA.

Quick Overview for ADAM10 antibody (C-Term) (ABIN499211)

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM10 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-ADAM10 Antibody

Binding Specificity: C-Term

Specificity: ADAM10 antibody was raised against a peptide corresponding to amino acids 732 to 748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid (2,4).

Purification: Ion exchange chromatography

Immunogen: Human ADAM10 (C-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: ADAM10 can be used for detection of ADAM10 at 1: 500 to 1: 2000 dilution. Anapproximately 85 kDa band can be detected, which may represent precursor. Immunocytochemistry: 2,0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: CD156c / ADAM10

Background: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-alpha, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).Synonyms: Disintegrin and metalloproteinase domain-containing protein 10, KUZ, Kuzbanian protein homolog, MADM, Mammalian disintegrin-metalloprotease

Gene ID: 102

NCBI Accession: NP_001101

UniProt: O14672

Pathways: Notch Signaling, EGFR Signaling Pathway