ACCN5 antibody (Extracellular)

Catalog No. ABIN7042919

Product Details anti-ACCN5 Antibody

Target: ACCN5 (Amiloride-Sensitive Cation Channel 5, Intestinal (ACCN5))

Binding Specificity: AA 284-297, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACCN5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to ASIC5 Channel

Specificity: Extracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat,human - 13,14 amino acid residues identical

Characteristics: Anti-ASIC5 (extracellular) Antibody (ABIN7042919, ABIN7045260 and ABIN7045261) is a highly specific antibody directed against an epitope of the mouse protein. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize ASIC5 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RQLKTVHQEYPWGE, corresponding to amino acid residues 284-297 of mouse ASIC5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234716)

Blocking Peptide: (ABIN7234716)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ACCN5

Target: ACCN5 (Amiloride-Sensitive Cation Channel 5, Intestinal (ACCN5))

Alternative Name: ASIC5 (ACCN5 Products)

Background: Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5,ASIC5 (acid sensing ion channel subunit family member 5) is an amiloride-sensitive ion channel belonging to the Degenerin/Epithelial Na+ channel (Deg/ENaC) family, a group of voltage-insensitive cation channels expressed in the nervous system and in several types of epithelial and immune cells.Structurally, ASICs have two transmembrane domains which line the pore of the channel with a relatively short cytoplasmic section, intracellular N- and C-termini and a large extracellular loop. The proton ligand binds to the extracellular domain, composed of 12 β sheets and 7 α helices2,3.ASICs are permeable to sodium, potassium, and sometimes to calcium and are activated by low pH , both intracellular and extracellular. They are responsible for conducting depolarizing inward Na+ currents. ASICs also contribute to excitotoxic neuronal damage during ischemia and local acidosis due to their sensitivity to acid1-3.ASICs are abundantly expressed in mammalian neurons. ASIC5 is mainly expressed in the brain in the cerebellum, specifically in the ventral uvula and nodulus of the vestibulocerebellum. ASIC5 mRNA is also detected in the liver mainly in the luminal membrane of epithelial cholangiocytes that line the bile duct1.ASICs have been linked to numerous neurological diseases including Parkinson's disease, stroke, traumatic brain injury, multiple sclerosis, and epilepsy3.

Alternative names: ASIC5, Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5

Gene ID: 58170

NCBI Accession: NM_017419

UniProt: Q9R0Y1