Aquaporin 1 antibody (Intracellular)

Catalog No. ABIN7042932

This anti-Aquaporin 1 antibody is a Rabbit Polyclonal antibody detecting Aquaporin 1 in WB, IHC and IF. Suitable for Human.

Quick Overview for Aquaporin 1 antibody (Intracellular) (ABIN7042932)

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-Aquaporin 1 Antibody

Binding Specificity: AA 242-260, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 1 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: mouse,toad (Bufo marinus) - 14, frog (Rana esculenta),Rat,19 amino acid residues identical,cattle, dog - 18,sheep - identical

Characteristics: Anti-Aquaporin 1 Antibody (ABIN7042932, ABIN7045194 and ABIN7045195) is a highly specific antibody directed against an epitope of the human AQP1 channel. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KVWTSGQVEEYDLDADDIN corresponding to amino acid residues 242-260 of human AQP1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: IHC|Whole Tissue Staining

Negative Control: (ABIN7234751)

Blocking Peptide: (ABIN7234751)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Alternative Name: AQP1

Background: AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule,Aquaporin 1 (AQP-1) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-1 together with AQP-2, AQP-4 and AQP-5 belongs to the first group1.Little is known about the function of the two newest members, AQP-11 and AQP-12.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.AQP-1 is widely expressed in several organs with prominent expression found in kidney, lung, red blood cells intestine and brain. Studies with mice lacking AQP-1 show that the channel has a critical role in urine concentration2. In addition, AQP-1 expression in tumor cells was shown to contribute to enhanced tumor spread and metastatic potential3.

Alternative names: Aquaporin 1, AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule

Gene ID: 358

NCBI Accession: NM_198098

UniProt: P29972

Pathways: Hormone Transport