CACNG1 antibody (Extracellular)

Catalog No. ABIN7042988

Product Details anti-CACNG1 Antibody

Target: CACNG1 (Calcium Channel, Voltage-Dependent, gamma Subunit 1 (CACNG1))

Binding Specificity: AA 30-44, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to CACNG1 (CaVγ1) Subunit

Specificity: 1st extracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-CACNG1 (extracellular) Antibody is directed against an epitope of rat CaVγ1. Anti-CACNG1 (extracellular) Antibody (ABIN7042988, ABIN7044053 and ABIN7044054) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize CaVγ1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DHWAVLSPHLEHHNE, corresponding to amino acid residues 30-44 of rat CACNG1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7234934)

Blocking Peptide: (ABIN7234934)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CACNG1

Target: CACNG1 (Calcium Channel, Voltage-Dependent, gamma Subunit 1 (CACNG1))

Alternative Name: CACNG1 (CACNG1 Products)

Background: CaVγ1, CACNLG, Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle Ca2+ channel subunit gamma,Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+conducting pores in the plasma membrane1. On the basis of their voltage activation properties, the voltage-gated Ca2+ channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels.2. CaV channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3.CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. The γ subunit is an integral membrane protein. The γ subunit family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5.CaVγ1 is solely expressed in skeletal muscle and associates with the α1S (CaV1.1) subunit6.Knockout mice are indeed viable and show no extreme phenotype. It seems that γ1 enhances the inactivation of ER Ca2+ release in a voltage-dependent manner6.

Alternative names: CACNG1, CaVgamma1, CACNLG, Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle Ca2+ channel subunit gamma

Gene ID: 29658

NCBI Accession: NM_000727

UniProt: P97707