CACNG5 antibody (Extracellular)

Catalog No. ABIN7042993

Product Details anti-CACNG5 Antibody

Target: CACNG5 (Calcium Channel, Voltage-Dependent, gamma Subunit 5 (CACNG5))

Binding Specificity: AA 87-99, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunochromatography (IC), Immunofluorescence (IF), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to CACNG5 (CaVγ5) Subunit

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-CACNG5 (extracellular) Antibody is directed against an extracellular epitope of rat CaVγ5. Anti-CACNG5 (extracellular) Antibody (ABIN7042993, ABIN7044061 and ABIN7044062) can be used in western blot and live cell imaging experiments. It has been designed to recognize CaVγ5 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SESTVNVLKMIRS, corresponding to amino acid residues 87-99 of rat CACNG5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:1000

Comment:

Negative Control: (ABIN7234950)

Blocking Peptide: (ABIN7234950)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CACNG5

Target: CACNG5 (Calcium Channel, Voltage-Dependent, gamma Subunit 5 (CACNG5))

Alternative Name: CACNG5 (CACNG5 Products)

Background: CaVγ5, Voltage-dependent calcium channel gamma-5 subunit, TARP gamma-5, Transmembrane AMPAR regulatory protein gamma-5,Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. On the basis of their voltage activation properties, the voltage-gated Ca2+ channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels.2. CaV channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3.CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. The γ subunit is an integral membrane protein. The γ subunit family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5.The CaVγ5 subunit is highly expressed in the liver, kidney, heart, lung, skeletal muscles, and, with a lower abundance, in testes5. The close association of epilepsy and ataxia with mutations in other neuronal voltage-dependent Ca2+ channels suggests these are potential candidate phenotypes for defects in the CaVγ5 (CACNG5) gene6.

Alternative names: CACNG5, CaVgamma5, Voltage-dependent calcium channel gamma-5 subunit, TARP gamma-5, Transmembrane AMPAR regulatory protein gamma-5

Gene ID: 140726

UniProt: Q8VHW8