CALHM1 antibody (Intracellular) (Atto 594)

Catalog No. ABIN7043014

Product Details anti-CALHM1 Antibody (Atto 594)

Target: CALHM1 (Calcium Homeostasis Modulator 1 (CALHM1))

Binding Specificity: AA 252-265, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CALHM1 antibody is conjugated to Atto 594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Calcium Homeostasis Modulator 1 Conjugated to the Fluorescent Dye ATTO-594

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-CALHM1 Antibody (ABIN7043013, ABIN7044044 and ABIN7044045) is a highly specific antibody directed against an epitope of the human calcium homeostasis modulator protein 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CALHM1 from rat, mouse, and human samples. \nAnti-CALHM1-ATTO Fluor-594 Antibody (ABIN7043014) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CALHM1-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human CALHM1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7235005)

Blocking Peptide: (ABIN7235005)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CALHM1

Target: CALHM1 (Calcium Homeostasis Modulator 1 (CALHM1))

Alternative Name: CALHM1 (CALHM1 Products)

Background: Calcium homeostasis modulator 1, FAM26C,A misbalance in Ca2+ homeostasis seems to be related to the development of Alzheimer's disease (AD). Indeed Neuronal Ca2+ balance may affect the levels of proteins associated with AD, such as amyloid-beta (Ab) and tau1.Calcium homeostasis modulator 1 (CALHM1) is a cerebral Ca2+ channel component responsible for controlling intracellular Ca2+ levels and Ab metabolism2,3. CALHM1 is a three transmembrane glycoprotein, mostly localized to the endoplasmic reticulum (ER) although it can be detected at the plasma membrane. A functional channel is formed by multiple subunits which has some structural similarities to the NMDA receptor. It is expressed in all brain regions1.In cultured cells, overexpression of CALHM1 increases intracellular Ca2+ levels and reduces Ab accumulation, thereby reinforcing its implication in the development of AD. Indeed, a polymorphism in the gene was found to affect the onset of AD2.

Alternative names: CALHM1, Calcium homeostasis modulator 1, FAM26C

Gene ID: 255022

NCBI Accession: NM_001001412

UniProt: Q8IU99