NBC4 antibody (Intracellular)

Catalog No. ABIN7043118

Product Details anti-NBC4 Antibody

Target: NBC4 (Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4))

Binding Specificity: AA 11-23, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NBC4 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to NBC4 Transporter

Specificity: Intracellular, N-terminus

Cross-Reactivity (Details): Not recommended for human and mouse samples.

Predicted Reactivity: Rat only

Characteristics: Anti-Rat SLC4A5 (NBC4) Antibody is directed against an epitope of rat NBCe2. Anti-Rat SLC4A5 (NBC4) Antibody (ABIN7043118, ABIN7044819 and ABIN7044820)) can be used in western blot analysis. It has been designed to recognize NBCe2 from rat samples. The antibody is not recommended for mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KLEPTSYRRRHPE, corresponding to amino acid residues 11-23 of rat NBCe2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: IFC

Negative Control: BLP-NT076

Blocking Peptide: BLP-NT076

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NBC4

Target: NBC4 (Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4))

Alternative Name: SLC4A5 (NBC4 Products)

Background: Electrogenic sodium bicarbonate cotransporter 4, NBCe2,The SLC4 transporter family, also known as the bicarbonate (HCO3-)-transporter family, includes the products of ten human genes (SLC4A1-5, A7-11). In mammals, the SLC4 membrane proteins are critical for several physiological processes, including the carriage of carbon dioxide (CO2) from the systemic capillaries to the pulmonary capillaries, the regulation of cell volume in multiple cell types, and the regulation of intracellular pH in nearly every cell of the body1.NBCe2 (NBC4) is an electrogenic sodium bicarbonate co-transporter encoded by SLC4A5. There are six NBCe2 isoform (SLC4A5A-F) reported2. NBCe2 is an integral membrane protein consisting of 14 transmembrane domains. NBCe2 has a long N-terminal hydrophilic domain and a much shorter C-terminal hydrophilic domain, both of which are intracellular3.NBCe2 is predominantly detected in the brain (highest in prefrontal cortex), epididymis, cardiac muscle, smooth muscle, kidney, and choroid plexus/apical1.Several studies identified SLC4A5 as a positional candidate for hypertension4,5. In mice, disruption of slc4a5 causes both arterial hypertension as well as metabolic acidosis (distal renal tubular acidosis)6. In addition, mice lacking NBCe2 have reduced choroid plexus secretion and reduced ventricle size. These combined issues change intracranial pressure, CSF electrolytes, and cause severe eye pathologies such as photoreceptor loss, ganglion cell loss, and retinal detachment7.

Alternative names: SLC4A5 (NBC4), Electrogenic sodium bicarbonate cotransporter 4, NBCe2

Gene ID: 297386

NCBI Accession: NM_001386136

UniProt: Q6RI88