EPH Receptor B4 antibody (Extracellular)

Catalog No. ABIN7043127

Product Details anti-EPH Receptor B4 Antibody

Target: EPH Receptor B4 (EPHB4)

Binding Specificity: AA 472-486, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to EphB4 Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-EphB4 (extracellular) Antibody (ABIN7043127, ABIN7044253 and ABIN7044254)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize EphB4 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HEKGAEGPSSVRFLK, corresponding to amino acid residues 472-486 of mouse EphB4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-ER024

Blocking Peptide: BLP-ER024

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for EPH Receptor B4

Target: EPH Receptor B4 (EPHB4)

Alternative Name: EPHB4 (EPHB4 Products)

Background: Ephrin type-B receptor 4, Eph receptor B4, Hepatoma transmembrane kinase, HTK,EphB4 (Ephrin type-B receptor 4) is a member of the Eph family of receptor tyrosine kinases. Signaling pathways of these receptors play important roles in regulating numerous physiological processes during development including cell morphology, proliferation, migration and axonal guidance, dendritic spine formation and neural plasticity. Ephrins transduce bidirectional signals based on their distribution on the communicating cell surfaces. Eph receptors mediate forward signaling in one cell while ephrin ligands transmit reverse signaling in an adjacent cell1,2.Eph receptors consist of two large subfamilies: EphAs and EphBs that bind to either glycophosphatidylinositol (GPI)-anchored ephrin-A ligands or transmembrane ephrin-B ligands respectively. Each eph receptor may interact with multiple ligands, except for EPHB4, which only binds ephrin-B22.EPHB4 is expressed primarily during embryonic development in neural tissue and immature vasculature. Studies show that EphB4 is implicated in the growth of several epithelial tumors and is a promising target in cancer therapy. EphB4 signaling was found to promote tumor growth, invasiveness, chemoresistance and tumor angiogenesis3.

Alternative names: EphB4, Ephrin type-B receptor 4, Eph receptor B4, Hepatoma transmembrane kinase, HTK

Gene ID: 13846

NCBI Accession: NM_004444

UniProt: P54761

Pathways: RTK Signaling