FSHR antibody (Extracellular)

Catalog No. ABIN7043134

Product Details anti-FSHR Antibody

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Binding Specificity: AA 128-140, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FSHR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to FSH Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,human - 12,13 amino acid residues identical

Characteristics: Anti-FSHR (extracellular) Antibody is directed against an extracellular epitope of the rat FSH receptor. Anti-FSHR (extracellular) Antibody (ABIN7043134, ABIN7044277 and ABIN7044278) can be used in western blot and live cell imaging applications. It has been designed to recognize the FSH receptor from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SNTGIKHLPAVHK, corresponding to amino acid residues 128-140 of rat FSHR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235325)

Blocking Peptide: (ABIN7235325)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for FSHR

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Alternative Name: FSHR (FSHR Products)

Background: FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO,FSH (follicle stimulating hormone) is the central hormone of mammalian reproduction, necessary for gonadal development and maturation at puberty and for gamete production during the fertile phase of life1. Together with LH, this gonadotropin is produced and secreted by the pituitary gland as a highly heterogeneous glycoprotein2. The FSH receptor belongs to the family of G-protein coupled receptors, complex transmembrane proteins characterized by seven hydrophobic helices inserted in the plasma membrane and by intracellular and extracellular domains of variable dimensions depending on the type of ligand3. The intracellular portion of the FSH receptor is coupled to a Gs protein and, upon receptor activation by the hormonal interaction with the extracellular domain it initiates a cascade of events that ultimately leads to the specific biological effects of the gonadotropin.FSH acts by binding to specific receptors, localized exclusively in the gonads. The FSH receptor is expressed by two cell types of the gonad, namely Sertoli cells in the testis, and granulosa cells in the ovarian follicle4.Mutations of FSHR may cause primary or secondary amenorrhea, infertility, premature ovarian failure (POF) and ovarian hyperstimulation syndrome (OHSS)5. Mutations of FSHR may also play a role in the development of granulosa cell tumors of ovaries6.

Alternative names: FSHR, FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO

Gene ID: 25449

NCBI Accession: NM_000145

UniProt: P20395

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Platelet-derived growth Factor Receptor Signaling