FFAR1 antibody (Extracellular)

Catalog No. ABIN7043137

Product Details anti-FFAR1 Antibody

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Binding Specificity: AA 244-259, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FFAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 1 (GPR40)

Specificity: 3rd extracellular loop

Predicted Reactivity: 16 amino acid residues identical,Mouse - 15, human - 12

Characteristics: Anti-GPR40/FFAR1 (extracellular) Antibody (ABIN7043137, ABIN7044279 and ABIN7044280)) is a highly specific antibody directed against an epitope of the rat Free fatty acid receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize FFAR1 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NVASFINPDLEGSWRK corresponding to amino acid residues 244-259 of rat FFAR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-FR011

Blocking Peptide: BLP-FR011

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for FFAR1

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Alternative Name: FFAR1 (FFAR1 Products)

Background: G-protein coupled receptor 40, Free fatty acid receptor 1,Free fatty acids (FFAs) were for long believed to influence cellular metabolism. Hoever, the discovery that FFAs actually mediate their effects through one of three G-protein coupled receptors (GPCRs) - Free Fatty Acid Receptors 1-3 (FFAR1-3), has initiated a number of studies in order to assess their implication in health and disease1.Like all other GPCRs, FFAR1-3 have seven transmembrane domains, an extracellular N-terminal tail and an intracellular C-terminus.FFAR1 is activated by either medium or long, saturated or unsaturated fatty acids2,3, whereas FFAR2 and FFAR3 are activated by short chain fatty acids4,5. FFAR1 is coupled to Gq and leads to Ca2+ mobilization upon activation. FFAR2 couples to both pertussis-sensitive and insensitive G-proteins and also leads to increased intracellular Ca2+ levels. FFAR3 coupling to G-protein induces an increase in cAMP1.Distribution studies of all three FFA Receptors indicate that FFAR1 mRNA is quite elevated in the pancreas2,3. FFAR1 is also detected in the liver, heart, skeletal muscle, and brain, although this broad localization pattern is supported only by one study2. FFAR2 is expressed in many tissues like liver and colon6. FFAR3 is expressed in many tissues but shows highest expression in adipose tissue4. Relatively high expression of the receptor could be detected in pancreas, spleen, lymph nodes and bone marrow4,5.As mentioned above, the finding that FFAs' actions are mediated by GPCRs, prompted many studies in order to elucidate their roles in healthy and disease states. Such studies stipulate that FFAR1 may be involved in obesity and type 2 diabetes since circulating free fatty acid levels in the plasma are significantly elevated. On the other hand, FFAR2 and FFAR3 could be potential targets for treating irritable bowel disease and appetite control1.

Alternative names: GPR40/FFAR1, G-protein coupled receptor 40, Free fatty acid receptor 1

Gene ID: 266607

NCBI Accession: NM_005303

UniProt: Q8K3T4

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Carbohydrate Homeostasis