FFAR1 antibody (Internal Region)

Catalog No. ABIN6261716

This Rabbit Polyclonal antibody specifically detects FFAR1 in WB and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for FFAR1 antibody (Internal Region) (ABIN6261716)

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FFAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FFAR1 Antibody

Binding Specificity: Internal Region

Purpose: GPR40 Antibody

Specificity: GPR40 Antibody detects endogenous levels of total GPR40.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GPR40, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for FFAR1

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Alternative Name: GPR40

Background:

Description: G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1). May also play a role in bone homeostasis, receptor signaling activates pathways that inhibit osteoclast differentiation (By similarity). Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G(q) and G(i)-mediated pathway.

Gene: FFAR1

Molecular Weight: 31 kDa

Gene ID: 2864

UniProt: O14842

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Carbohydrate Homeostasis