GPR174 antibody (Extracellular)

Catalog No. ABIN7043151

Product Details anti-GPR174 Antibody

Target: GPR174 (G Protein-Coupled Receptor 174 (GPR174))

Binding Specificity: AA 160-175, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR174 antibody is un-conjugated

Application: Western Blotting (WB), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to GPR174

Specificity: 2nd extracellular loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - 15,16 amino acid residues identical, mouse,rat - 13

Characteristics: Anti-GPR174 (extracellular) Antibody (ABIN7043151, ABIN7044455 and ABIN7044456)) is a highly specific antibody directed against an epitope of the human receptor. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize GPR174 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DTSGNRTK(S)FVDLPTR, corresponding to amino acid residues 160 - 175 of human GPR174

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-GR060

Blocking Peptide: BLP-GR060

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR174

Target: GPR174 (G Protein-Coupled Receptor 174 (GPR174))

Alternative Name: GPR174 (GPR174 Products)

Background: G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79,GPR174 (LysoPS, LYPSR3), is one of three lysophosphatidyl serine receptors, belonging to the superfamily of G-protein coupled receptors. Lysophospholipids are a diverse group of small, membrane-derived phospholipids that act as extracellular signals1.Activation of GPR174 leads to an increase in cAMP cellular levels, leading to cellular morphological changes, cell to cell adhesion, and delays in cell proliferation2.GPR174 is reported to be a genetic risk locus for Graves disease, an autoimmune disorder that causes hyperthyroidism3 and as a negative regulator of T-cells activation4.

Alternative names: G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79

Gene ID: 84636

NCBI Accession: NM_032553

UniProt: Q9BXC1