GPR37 antibody (Extracellular)

Catalog No. ABIN7043157

Product Details anti-GPR37 Antibody

Target: GPR37 (G Protein-Coupled Receptor 37 (Endothelin Receptor Type B-Like) (GPR37))

Binding Specificity: AA 234-247, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR37 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to GPR37

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-GPR37 (extracellular) Antibody (ABIN7043157, ABIN7044437 and ABIN7044438) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR37 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RVRLKNPFYPLTQE, corresponding to amino acid residues 234-247 of rat GPR37

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235404)

Blocking Peptide: (ABIN7235404)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR37

Target: GPR37 (G Protein-Coupled Receptor 37 (Endothelin Receptor Type B-Like) (GPR37))

Alternative Name: GPR37 (GPR37 Products)

Background: G-protein coupled receptor 37, Prosaposin receptor GPR37, Parkin-associated endothelin receptor-like receptor, Endothelin B receptor-like protein 1,GPR37 or parkin-associated endothelin-like receptor, is an orphan G-protein coupled receptor (GPCR) almost exclusively expressed in the central nervous system. High levels of the protein are detected in the cerebellum, corpus callosum, caudate nucleus, putamen, substantia nigra, and hippocampus.GPR37 is a substrate of the E3 ubiquitin ligase parkin and acts as a receptor for prosaposin. The protein affects oligodendrocyte differentiation and myelination during development1,2.Studies show that GPR37 is heavily involved in the pathogenesis of Parkinson's disease. Missense mutations of GPR37 substrate E3 ubiquitin ligase parkin result in the accumulation of GPR37 in the brain of Parkinson's disease patients1,2. In addition, GPR37 levels are up-regulated in the brains of juvenile Parkinson disease patients and down-regulated in the amygdala and hippocampus of major depressive disorder patients3.

Alternative names: GPR37, G-protein coupled receptor 37, Prosaposin receptor GPR37, Parkin-associated endothelin receptor-like receptor, Endothelin B receptor-like protein 1

Gene ID: 117549

NCBI Accession: NM_005302

UniProt: Q9QYC6