GPR37L1 antibody (Extracellular)

Catalog No. ABIN7043158

Product Details anti-GPR37L1 Antibody

Target: GPR37L1 (G Protein-Coupled Receptor 37 Like 1 (GPR37L1))

Binding Specificity: AA 66-80, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR37L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to GPR37L1 (extracellular)

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14 out of 15 amino acid residues identical

Characteristics: Anti-GPR37L1 (extracellular) Antibody (ABIN7043158, ABIN7044439 and ABIN7044440) is a highly specific antibody directed against an extracellular epitope of the rat GPR37L1. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR37L1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EYPKPIHPAGLQPTK, corresponding to amino acid residues 66 - 80 of rat GPR37L1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: (ABIN7235408)

Blocking Peptide: (ABIN7235408)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR37L1

Target: GPR37L1 (G Protein-Coupled Receptor 37 Like 1 (GPR37L1))

Alternative Name: GPR37L1 (GPR37L1 Products)

Background: G-Protein Coupled Receptor 37-Like 1, Endothelin B Receptor-Like Protein 2, ETBRLP2, Prosaposin Receptor GPR37L1,G prtoein coupled receptor 37L1 (GPR37L1), also known as Endothelin B Receptor-Like Protein 2, is an orphan receptor (a receptor without an assigned ligand yet) that shares structural homology with the endothelin receptors family of GPCRs. 1GPR37L1 is a 481 amino acid GPCR with 68 % similarity and 48 % identity to GPR37, another orphan endothelin receptor like GPR. In addition to the seven trans- membrane helixes typical for GPCRs, it has a cysteine cluster at the end of helix 8. 2GPR37L1 has been shown to bind the neuroprotective and glioprotective factor prosaposin (PSAP), although later studies did not confirm prosaposin as a functional ligand. 3GPR37L1 is highly expressed in the central nervous system, with some expression in the spinal cord, placenta, liver, stomach, and testis. 4,5GPR37L1 signaling pathway was shown to have an effect on motor activity, blood pressure regulation, cellular development and protection from cytotoxic agents. Deletion of the receptor caused marked developmental changes in the cerebellum, and hence the discovery of the native ligand for this receptor and studying the signaling pathway that is induced by it, is of high medicinal importance 1.

Alternative names: G-Protein Coupled Receptor 37-Like 1, Endothelin B Receptor-Like Protein 2, ETBRLP2, Prosaposin Receptor GPR37L1

Gene ID: 252939

NCBI Accession: NM_004767

UniProt: Q9QYC5