GABRA6 antibody (Extracellular)

Catalog No. ABIN7043190

This anti-GABRA6 antibody is a Rabbit Polyclonal antibody detecting GABRA6 in WB, IHC, IF, IC and LCI. Suitable for Rat.

Quick Overview for GABRA6 antibody (Extracellular) (ABIN7043190)

Target: GABRA6 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-GABRA6 Antibody

Binding Specificity: AA 20-37, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GABA(A) α6 Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 17,19 amino acid residues identical

Characteristics: Anti-GABA(A) alpha6 Receptor (extracellular) Antibody is directed against an extracellular epitope of the rat GABA(A) α6 receptor. Anti-GABA(A) α6 Receptor (extracellular) Antibody (ABIN7043190, ABIN7044301 and ABIN7044302) can be used in western blot, immunohistochemical and live cell imaging applications. It has been designed to recognize GABRA6 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: QLEDEGNFYSENVSRILDN(C), corresponding to amino acid residues 20-37 of rat GABRA6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235504)

Blocking Peptide: (ABIN7235504)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GABRA6

Target: GABRA6 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6))

Alternative Name: GABRA6

Background: γ-Aminobutyric acid receptor type A subunit α6, GABRA6,GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake and metabolism occur in the nervous system.1The GABA transmitter interacts with two major types of receptors: ionotropic GABA(A) and metabotropic GABA(B) receptors. The GABA(A) receptor belongs to the ligand gated ion channel superfamily.2The GABA(A) R, is a heteropentamer, with all of its five subunits contributing to the pore formation.To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ?.1 Six α subunit isoforms were described to exist in mammals (α1-α6). The native GABA(A) receptor, in most cases, consists of 2α, 2β and 1γ subunit. The α subunit is the most common subunit and is expressed ubiquitously. It determines the affinities for allosteric ligands shown by GABA(A)R.A possible role for the GABA(A) receptors in alcohol action has been derived from animal studies, in vitro cell models and human research which suggested an important role for GABA(A) α6 along with β2, α1 and γ2, in alcohol related phenotypes.3In Canavan disease (inherited leukodystrophy), low level of expression of GABA(A) α6 was reported.4

Alternative names: GABA(A) alpha6 Receptor, gamma-Aminobutyric acid receptor type A subunit alpha6, GABRA6

Gene ID: 29708

NCBI Accession: NM_000811

UniProt: P30191

Pathways: Synaptic Membrane